Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit Dx

Concentrate circulating DNA into a flexible elution volume ranging from (25 µL - 50 µL) Isolate inhibitor-free cell-free circulating DNA Compatible with Streck Cell-Free DNA BCT® Tubes Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Research use format is also available These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample. These kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS. Background Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitocondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia. Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit Dx For serum input volumes 10 µL - 200 µL. Purify high-quality DNA in 15-20 minutes Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit Dx For serum input volumes 200 µL - 500 µL. Purify high-quality DNA in 15-20 minutes. Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit Dx The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here to view. For serum input volumes 1 mL - 4 mL. Purify high-quality DNA in 90 minutes. Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit Dx The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here to view. For serum input volumes 5 mL - 10 mL. Two kits in one - purify and concentrate DNA from bodily fluids using a convenient two column system.