Endotoxin Removal Kit (Midi) - For DNA

Reduce endotoxin levels to 0.1 EU/µg DNA or less Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume Remove endotoxins from up to 1 mg of DNA with the Maxi format kit Fast and easy processing using a rapid spin-column format These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample. Endotoxin Removal Kit (Mini) This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes. Endotoxin Removal Kit (Midi) This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. Endotoxin Removal Kit (Maxi) This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. About Endotoxins Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.