H pylori Antibody (Catalase)

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Not Applicable

Host

Mouse

Reactivity

Helicobacter pylori

Immunogen

A portion of amino acids 323-445 from the Helicobacter pylori Catalase protein was used as the immunogen for the H pylori antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HPYL/7228

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the H pylori antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This H pylori antibody is available for research use only.

Storage Conditions

Aliquot the H pylori antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the H pylori antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE Helicobacter pylori-infected human stomach tissue with H pylori antibody (clone HPYL/7228) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.