Recombinant Prostein Antibody / SLC45A3

Human prostein is a 553 aa protein identified by cDNA library subtraction and subsequent high throughput microarray screening of prostate cancer. Prostein has multiple transmembrane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. By immunohistochemistry, prostein is expressed in the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in normal and malignant tissue samples representing the great majority of essential tissues and tumors. In particular, prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q96JT2

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide corresponding to the N terminus of the human protein was used as the immunogen for the recombinant Prostein antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SLC45A3/7176R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Prostein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Prostein antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Prostein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Prostein antibody should be determined by the researcher.

Location

Membrane, Vesicles, Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using recombinant Prostein antibody (clone SLC45A3/7176R) . These results demonstrate the foremost specificity of the SLC45A3/7176R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.