MED7 Antibody

MED7 (Mediator of RNA polymerase II transcription subunit 7), also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (use assay dependent concentration, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

O43513

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the MED7 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-MED7-1B8

Applications

ELISA, FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MED7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MED7 antibody is available for research use only.

Storage Conditions

Aliquot the MED7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MED7 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using MED7 antibody (clone PCRP-MED7-1B8) . These results demonstrate the foremost specificity of the PCRP-MED7-1B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.