HDAC3 Antibody

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

O15379

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human HDAC3 protein was used as the immunogen for the HDAC3 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-HDAC3-3C9

Applications

ELISA, WB, FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the HDAC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HDAC3 antibody is available for research use only.

Storage Conditions

Aliquot the HDAC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HDAC3 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using HDAC3 antibody (clone PCRP-HDAC3-3C9) . These results demonstrate the foremost specificity of the PCRP-HDAC3-3C9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.