CBF beta Antibody / CBFB

The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFB (core binding factor subunit beta) and AML3 (acute myeloid leukemia), is composed of two subunits and is essential for the regulation of hematopoiesis and osteogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

Q13951

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the CBF beta antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-CBFB-1E6

Applications

ELISA, WB, FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CBF beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CBF beta antibody is available for research use only.

Storage Conditions

Aliquot the CBF beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CBF beta antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using CBF beta antibody (clone PCRP-CBFB-1E6) . These results demonstrate the foremost specificity of the PCRP-CBFB-1E6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.