Ku80 Antibody / XRCC5
The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86) which is also known by the synonym Ku-80 or (p80) . Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Ku has several functions, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity; serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P13010
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 300-500 was used as the immunogen for the Ku80 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
XRCC5/7317
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Ku80 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Ku80 antibody is available for research use only.
Storage Conditions
Aliquot the Ku80 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Ku80 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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