MED22 Antibody
Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Immunofluorescence: 0.5-2 µg/mL, Flow cytometry: 0.5-2ug/million cells
UniProt
Q15528
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the MED22 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-MED22-2A7
Applications
ELISA, IF, FACS
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the MED22 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MED22 antibody is available for research use only.
Storage Conditions
Aliquot the MED22 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the MED22 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human human U-87 MG cells using MED22 antibody (green, clone PCRP-MED22-2A7) and phalloidin (red) .
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