ERK2 Antibody / MAPK1
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42) . Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 0.5-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P28482
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the ERK2 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-MAPK1-1D1
Applications
ELISA, FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the ERK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ERK2 antibody is available for research use only.
Storage Conditions
Aliquot the ERK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/erk2-antibody-mapk1-pcrp-mapk1-1d1-v9723
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the ERK2 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using ERK2 antibody (green, clone PCRP-MAPK1-1D1) and phalloidin (red) .
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