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Recombinant KRT20 Antibody / Cytokeratin 20

This MAb recognizes an intermediate filament protein of 46kDa, identified as CK20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P35900

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 324 424 was used as the immunogen for the recombinant KRT20 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT20/4380R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant KRT20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant KRT20 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant KRT20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant KRT20 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human colon carcinoma tissue with recombinant KRT20 antibody (clone KRT20/4380R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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