MICA Antibody

MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with -2-microglobulin. The expression is induced on proliferating or heat shock-stressed epithelial cells. MICA and MICB are broadly recognized by intestinal epithelial Î³Î´ T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q29983

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 was used as the immunogen for the MICA antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MICA/4443

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MICA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MICA antibody is available for research use only.

Storage Conditions

Aliquot the MICA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MICA antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE human stomach with MICA antibody (clone MICA/4443) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.