BNP Antibody / Brain Natriuretic Peptide / NPPB

Natriuretic peptides comprise a family of three structurally related molecules: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) . ANP and BNP act mainly as cardiac hormones, produced primarily by the atrium and ventricle, respectively, while the gene encoding C-type natriuretic peptide is expressed mainly in the brain. These peptides possess potent natriuretic, diuretic and vasodilating activities and are implicated in body fluid homeostasis and blood pressure control. ANP, BNP and CNP are highly homologous within the 17-residue ring structure formed by an intramolecular disulfide linkage. The genes which encode for ANP and BNP map to human chromosome 1p36.22. The gene which encodes for CNP maps to human chromosome 2q37.1.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16860

Host

Mouse

Reactivity

Human

Immunogen

A recombinant protein fragment was used as the immunogen for the BNP antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NPPB/4493

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the BNP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BNP antibody is available for research use only.

Storage Conditions

Aliquot the BNP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the BNP antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human uterus with BNP antibody (clone NPPB/4493) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.