Myf-4 Antibody / MYOG / Myogenin
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P15173
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the Myf-4 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
PCRP-MYOG-1C5
Applications
ELISA, FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Myf-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Myf-4 antibody is available for research use only.
Storage Conditions
Aliquot the Myf-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Myf-4 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using Myf-4 antibody (green, clone PCRP-MYOG-1C5) and phalloidin (red) .
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