PI3K Antibody (p85 beta) / PIK3R2

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85 heterotrimeric G proteins) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O00459

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 520-720 was used as the immunogen for the PI3K antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PIK3R2/292

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the PI3K antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PI3K antibody is available for research use only.

Storage Conditions

Aliquot the PI3K antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PI3K antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human bladder carcinoma tissue with PIK3 antibody (clone PIK3R2/292) . Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.