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LYZ Antibody / Lysozyme C

Lysozyme is an enzyme, commonly referred to as the body s own antibiotic since it kills bacteria. Natural substrate of lysozyme is the bacterial cell wall peptidoglycan (cleaving the beta[1-4] glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine) . Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P61626

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 18-147 was used as the immunogen for the LYZ antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

LYZ/3947

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the LYZ antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LYZ antibody is available for research use only.

Storage Conditions

Aliquot the LYZ antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the LYZ antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human spleen tissue with LYZ antibody (clone LYZ/3947) . Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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