TFIIB Antibody / GTF2B

In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells

UniProt

Q00403

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the TFIIB antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-GTF2B-1D1

Applications

ELISA, FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the TFIIB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TFIIB antibody is available for research use only.

Storage Conditions

Aliquot the TFIIB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TFIIB antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using TFIIB antibody (clone PCRP-GTF2B-1D1) . These results demonstrate the foremost specificity of the PCRP-GTF2B-1D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.