GTF2A1 Antibody / TFIIA
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor) . TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P52655
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the GTF2A1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-GTF2A1-1F2
Applications
ELISA, FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the GTF2A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GTF2A1 antibody is available for research use only.
Storage Conditions
Aliquot the GTF2A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the GTF2A1 antibody should be determined by the researcher.
Location
Nucleus, cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using GTF2A1 antibody (green, clone PCRP-GTF2A1-1F2) and phalloidin (red) .
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