CNOT10 Antibody / CCR4-NOT transcription complex subunit 10
CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
Q9H9A5
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the CNOT10 antibody.
Clonality
Monoclonal
Isotype
IgG2c
Clone
PCRP-CNOT10-1D5
Applications
ELISA, FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CNOT10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CNOT10 antibody is available for research use only.
Storage Conditions
Aliquot the CNOT10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CNOT10 antibody should be determined by the researcher.
Location
Cytoplasm (Mitochondrion) and Nucleus (PML body)
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using CNOT10 antibody (green, clone PCRP-CNOT10-1D5) and phalloidin (red) .
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