SIRT1 Antibody
The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent matingtype loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
Q96EB6
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the SIRT1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PCRP-SIRT1-1E11
Applications
ELISA, FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SIRT1 antibody is available for research use only.
Storage Conditions
Aliquot the SIRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SIRT1 antibody should be determined by the researcher.
Location
Cytoplasm, Mitochondrion and Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using SIRT1 antibody (green, clone PCRP-SIRT1-1E11) and phalloidin (red) .
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