SIRT3 Antibody

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA) . ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs) . These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC) .

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

Q9NTG7

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human SIRT3 protein was used as the immunogen for the SIRT3 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-SIRT3-1C10

Applications

ELISA, FACS, IF, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SIRT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SIRT3 antibody is available for research use only.

Storage Conditions

Aliquot the SIRT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SIRT3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of PFA-fixed human HeLa cells using SIRT3 antibody (green, clone PCRP-SIRT3-1C10) and phalloidin (red) .

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

APR05082G-01 50 µL

STX3 Antibody (Center) [APR05082G]

Ask

View Details

APR05082G-02 100 µL

STX3 Antibody (Center) [APR05082G]

Ask

View Details

APR05082G-03 200 µL

STX3 Antibody (Center) [APR05082G]

Ask

View Details

APR05082G-04 400 µL

STX3 Antibody (Center) [APR05082G]

Ask

View Details

TA808849AM 100 µL

SNX10 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D10]

Ask

View Details

sc-28366 AF488 200 µg/mL

Epac (A-5) Alexa Fluor® 488

Ask

View Details