ZHX3 Antibody

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA) . ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs) . These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC) .

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9H4I2

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human ZHX3 protein was used as the immunogen for the ZHX3 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-ZHX3-1G3

Applications

ELISA, FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ZHX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZHX3 antibody is available for research use only.

Storage Conditions

Aliquot the ZHX3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ZHX3 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thyroid tissue with ZHX3 antibody (clone PCRP-ZHX3-1G3) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.