SOX2 Antibody

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P48431

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human SOX2 protein was used as the immunogen for the SOX2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

SOX2/4267R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SOX2 antibody is available for research use only.

Storage Conditions

Aliquot the SOX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SOX2 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with SOX2 antibody (clone SOX2/4267R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.