Decapping Protein 2 Antibody / DCP2 / m7GpppN-mRNA hydrolase
The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
Q8IU60
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the Decapping Protein 2 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
PCRP-DCP2-1D6
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Decapping Protein 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Decapping Protein 2 antibody is available for research use only.
Storage Conditions
Aliquot the Decapping Protein 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Decapping Protein 2 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human MCF-7 using Decapping Protein 2 antibody (green, clone PCRP-DCP2-1D6) and phalloidin (red) .
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