Angiotensin Converting Enzyme Antibody / ACE

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P12821

Host

Mouse

Reactivity

Human

Immunogen

Full-length recombinant human protein was used as the immunogen for the Angiotensin Converting Enzyme antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ACE/3764

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Angiotensin Converting Enzyme antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Angiotensin Converting Enzyme antibody is available for research use only.

Storage Conditions

Aliquot the Angiotensin Converting Enzyme antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Angiotensin Converting Enzyme antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human colon carcinoma tissue with Angiotensin Converting Enzyme antibody (clone ACE/3764) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.