Recombinant Human Nuclear Antigen Antibody
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL
UniProt
Not Known
Host
Rabbit
Reactivity
Human
Immunogen
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
235-1R
Type
Recombinant
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Human Nuclear Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Human Nuclear Antigen antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Human Nuclear Antigen antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Immunofluorescent staining of MeOH-fixed human HeLa cells with recombinant Human Nuclear Antigen antibody (green, clone 235-1R) .
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