Recombinant VIM Antibody / Vimentin
When used in conjunction with anti-keratin staining, anti-vimentin staining is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P08670
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 366-466 was used as the immunogen for the recombinant VIM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RVIM/6914
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant VIM antibody is available for research use only.
Storage Conditions
Aliquot the recombinant VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant VIM antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6914) . Predicted molecular weight ~53 kDa.
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