Recombinant Vinculin Antibody
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Product Specifications
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P18206
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 174-322 was used as the immunogen for the recombinant Vinculin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
VCL/7091R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Vinculin antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Vinculin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/recombinant-vinculin-antibody-vcl7091r-v9615
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Vinculin antibody should be determined by the researcher.
CAS Number
9000-83-3
Location
Cytoplasm
Image Legend
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