KLF17 Antibody

Kruppel-like factors (KLFs) comprise a family of evolutionarily conserved zinc finger-containing transcription factors with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. Individual members of the Sp1-like/KLF family can function either as activators or repressors, depending on which promoter they bind and which co-regulators they interact with. KLF17 (Kruppel-like factor 17), whose alternative names include ZNF393 (zinc finger protein 393) or zfp393, is a 389 amino acid nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family. Expressed in testis and ovary, KLF17 may function as a germ cell-specific transcription factor involved in oocyte development and spermatid differentiation. Containing three C2H2-type zinc fingers which bind G/C-rich sites, KLF17 activates transcription from CACCC-box elements.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells

UniProt

Q5JT82

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KLF17protein was used as the immunogen for the KLF17 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-KLF17-1G2

Applications

WB, IF, FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the KLF17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KLF17 antibody is available for research use only.

Storage Conditions

Aliquot the KLF17 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the KLF17 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using KLF17 antibody (clone PCRP-KLF17-1G2) . These results demonstrate the foremost specificity of the PCRP-KLF17-1G2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.