DMRT2 Antibody

The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene (s) on chr 9. Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Western blot: 1-2 µg/mL

UniProt

Q9Y5R5

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human DMRT2 protein was used as the immunogen for the DMRT2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-DMRT2-1B11

Applications

FACS, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the DMRT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DMRT2 antibody is available for research use only.

Storage Conditions

Aliquot the DMRT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the DMRT2 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using DMRT2 antibody (clone PCRP-DMRT2-1B11) . These results demonstrate the foremost specificity of the PCRP-DMRT2-1B11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.