IL1 beta Antibody / IL1B

The IL1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE) . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01584

Host

Mouse

Reactivity

Human

Immunogen

A recombinant protein fragment was used as the immunogen for the IL1B antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

IL1B/3993

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the IL1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IL1B antibody is available for research use only.

Storage Conditions

Aliquot the IL1B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the IL1B antibody should be determined by the researcher.

Location

Cytoplasm, Extracellular (secreted)

Image Legend

IHC staining of FFPE human adrenal gland with IL1B antibody (clone IL1B/3993) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.