Osteopontin Antibody / OPN / SPP1
Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P10451
Host
Rat
Reactivity
Human
Immunogen
Recombinant full-length rat Spp1/Osteopontin protein was used as the immunogen for the Osteopontin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
OSP/4589
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Osteopontin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Osteopontin antibody is available for research use only.
Storage Conditions
Aliquot the Osteopontin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Osteopontin antibody should be determined by the researcher.
Location
Secreted
Image Legend
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