Superoxide Dismutase 1 Antibody / SOD1
Cu-Zn superoxide dismutase-1 (SOD-1) is a well-characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer s disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P00441
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the Superoxide Dismutase 1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SOD1/3923
Applications
FACS, IF, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Superoxide Dismutase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Superoxide Dismutase 1 antibody is available for research use only.
Storage Conditions
Aliquot the Superoxide Dismutase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Superoxide Dismutase 1 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of PFA-fixed human MCF-7 cells Superoxide Dismutase 1 antibody (green, clone SOD1/3923) and phalloidin (red) .
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