Osteonectin Antibody / SPARC

SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P09486

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 was used as the immunogen for the Osteonectin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

OSTN/3758

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Osteonectin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Osteonectin antibody is available for research use only.

Storage Conditions

Aliquot the Osteonectin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Osteonectin antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human tonsil tissue with Osteonectin antibody (clone OSTN/3758) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.