RPA32 Antibody / RPA2 / Replication Protein A2

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15927

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length Replication Protein A2 protein was used as the immunogen for the RPA32 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

RPA2/4774

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the RPA32 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RPA32 antibody is available for research use only.

Storage Conditions

Aliquot the RPA32 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the RPA32 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human tonsil tissue with RPA32 antibody (clone RPA2/4774) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.