Recombinant KRT7 Antibody / Cytokeratin 7

Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P08729

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 was used as the immunogen for the recombinant KRT7 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT7/4387R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant KRT7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant KRT7 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant KRT7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant KRT7 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human salivary gland tissue with recombinant KRT7 antibody (clone KRT7/4387R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.