Recombinant p27Kip1 Antibody / p27 / CDKN1B

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P46527

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant full-length human CDKN1B protein was used as the immunogen for the recombinant p27Kip1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

KIP1/1355R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant p27Kip1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant p27Kip1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human bladder tissue with recombinant p27Kip1 antibody (clone clone KIP1/1355R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. Strong nuclear staining observed.