NM23 Antibody / NME1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP) . In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15531

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human NME1 protein was used as the immunogen for the NM23 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

NME1/2738

Applications

ELISA, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the NM23 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NM23 antibody is available for research use only.

Storage Conditions

Aliquot the NM23 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NM23 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human breast carcinoma tissue with NM23 antibody (clone NME1/2738) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.