Vitamin D binding protein Antibody / VDBP / GC

Vitamin D-binding protein (DBP) is a multi-functional serum protein that binds to the plasma membranes of numerous cell types and mediates a variety of cellular functions. The locus of the DBP protein (also known as group-specific component protein or GC) is located at human chromosome 4q13.3. DBP functions in organ-specific transportation of vitamin D and its metabolites to the various target organs of the vitamin D endocrine system. In addition, DBP has immunomodulatory properties and is able to bind to the surface of leukocytes. DBP binds to the plasma membrane through a chondroitin sulfate proteoglycan. DBP serves as a co-chemotactic factor for C5a to enhance the chemotactic activity of C5a. DBP can also bind to globular Actin with high affinity and is involved in the clearance of Actin from the blood. DBP plays an important role in osteoclast differentiation. The diverse cellular functions of DBP require its cell surface binding ability to mediate different biological processes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P02774

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 35-175 was used as the immunogen for the Vitamin D binding protein antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

VDBP/4482

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Vitamin D binding protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Vitamin D binding protein antibody is available for research use only.

Storage Conditions

Aliquot the Vitamin D binding protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Vitamin D binding protein antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human pancreatic tissue with Vitamin D binding protein antibody (clone VDBP/4482) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.