FAP Antibody / Fibroblast Activation Protein Alpha

FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is colocalized with MMP-1 and MMP-13 as well as CD44 (variants v3 and v7/8) . Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q12884

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 was used as the immunogen for the FAP antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

FAP/4854

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the FAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FAP antibody is available for research use only.

Storage Conditions

Aliquot the FAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the FAP antibody should be determined by the researcher.

Location

Cell surface, Cell membrane

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using FAP antibody (clone FAP/4854) . These results demonstrate the foremost specificity of the FAP/4854 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.