Fibroblast Activation Protein Alpha Antibody / FAP

FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is colocalized with MMP-1 and MMP-13 as well as CD44 (variants v3 and v7/8) . Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q12884

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 was used as the immunogen for the Fibroblast Activation Protein Alpha antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

FAP/4853

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Fibroblast Activation Protein Alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Fibroblast Activation Protein Alpha antibody is available for research use only.

Storage Conditions

Aliquot the Fibroblast Activation Protein Alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Fibroblast Activation Protein Alpha antibody should be determined by the researcher.

Location

Cell surface, Cell membrane

Image Legend

IHC staining of FFPE human liver tissue with Fibroblast Activation Protein antibody (clone FAP/4853) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.