Recombinant Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1

ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P00352

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 315-434 was used as the immunogen for the recombinant Aldehyde Dehydrogenase 1A1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ALDH1A1/7011R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Aldehyde Dehydrogenase 1A1 ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Aldehyde Dehydrogenase 1A1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Aldehyde Dehydrogenase 1A1 ALDH1A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Aldehyde Dehydrogenase 1A1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R) . Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.