P27Kip1 Antibody / p27 / CDKN1B

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P46527

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CDKN1B protein was used as the immunogen for the p27Kip1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KIP1/1357

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This p27Kip1 antibody is available for research use only.

Storage Conditions

Aliquot the p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the p27Kip1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human colon tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.