ALPP Antibody / Placental Alkaline Phosphatase
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P05187
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human ALPP protein was used as the immunogen for the ALPP antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ALPP/4109
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the ALPP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ALPP antibody is available for research use only.
Storage Conditions
Aliquot the ALPP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ALPP antibody should be determined by the researcher.
Location
Cytoplasmic, Cell surface
Image Legend
Frequently Asked Questions
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