SERPINA3 Antibody / AACT / Alpha-1-Antichymotrypsin

It recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT) . AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01011

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids A portion of amino acids 49-187 was used as the immunogen for the SERPINA3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SERPINA3/4190

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SERPINA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SERPINA3 antibody is available for research use only.

Storage Conditions

Aliquot the SERPINA3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SERPINA3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human liver tissue with SERPINA3 antibody (clone SERPINA3/4190) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.