Recombinant CD3e Antibody / CD3 epsilon
CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA: 2-4 µg/mL (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P07766
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids aa 20-40 from the human protein was used as the immunogen for the recombinant CD3e antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
C3e/4652R
Type
Recombinant
Applications
ELISA, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CD3e antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CD3e antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
Location
Cell surface, Cytoplasm
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
PKD1L2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
