Recombinant Beta Tubulin Antibody / TUBB3

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q13509

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 437-450 from the human protein was used as the immunogen for the recombinant Beta Tubulin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

TUBB3/7089R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Beta Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Beta Tubulin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Beta Tubulin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Beta Tubulin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with recombinant Beta Tubulin antibody (clone TUBB3/7089R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.