Recombinant CD3 epsilon Antibody
Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains: epsilon, gamma, delta and zeta. The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR) . CD3 antigen appears at all stages of T cell development which makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias) .
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P07766
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 100-200 was used as the immunogen for the recombinant CD3 epsilon antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
C3e/4653R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CD3 epsilon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CD3 epsilon antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CD3 epsilon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD3 epsilon antibody should be determined by the researcher.
Location
Cell Surface, Cytoplasm
Image Legend
Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R) . Predicted molecular weight ~23 kDa.
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