Recombinant Sarcomeric Alpha Actinin Antibody / Alpha Actinin 2 / ACTN2

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P35609

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 557-692 from the human protein was used as the immunogen for the recombinant Sarcomeric Alpha Actinin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ACTN2/7041R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Sarcomeric Alpha Actinin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Sarcomeric Alpha Actinin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Sarcomeric Alpha Actinin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Sarcomeric Alpha Actinin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skeletal muscle with recombinant Sarcomeric Alpha Actinin antibody (clone ACTN2/7041R) . Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.