Recombinant Actin Antibody / ACTA2 / Smooth Muscle
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P62736
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full-length human ACTA2 protein was used as the immunogen for the recombinant Actin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ACTA2/1614R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Actin antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant Actin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Bovine Phospholipase A2 Receptor 1 (PLA2R1) ELISA Kit
Bovine Phospholipase A2 Receptor 1 (PLA2R1) ELISA Kit
Bovine Phospholipase A2 Receptor 1 (PLA2R1) ELISA Kit
Bovine Phospholipase A2 Receptor 1 (PLA2R1) ELISA Kit
